| Título: | Biofilm formation ability and genotypic analysis of Streptococcus uberis isolated from bovine mastitis |
| Autores: | Melina Vanesa Moliva, Autor ; Mirta Cristina Lasagno, Autor ; Carina Porporatto, Autor ; Elina Beatriz Reinoso, Autor |
| Tipo de documento: | documento electrónico |
| Editorial: | International Journal of Veterinary and Dairy Sciences, 2017 |
| Dimensiones: | 6 p. / il. byn. |
| Langues: | Inglés |
| Materias: |
02 - Temático General - UNESCO Enfermedad animal ; Genética animal ; Veterinaria ; Zootecnia |
| Etiquetas: | Streptococcus uberis ; mastitis ; biofilm ; biofilm related genes ; Pulse Field Gel Electrophoresis ; genes relacionados con el biofilm ; Electroforesis en gel de campo pulsado |
| Resumen: |
Bovine mastitis is an important problematic in the dairy industry all over the world. Streptococcus uberis is a prevalent environmental pathogen implicated in bovine mastitis able to produce biofilm. The aim of this study was assay 28 S. uberis collected from cows with mastitis in Argentina in respect to their biofilm formation ability. Detection of biofilm related genes and genetic relationships by Pulse Field Gel Electrophoresis among the isolates were evaluated to determinate correlation with biofilm formation ability. Microtiter plate assay revealed that all of isolates produced biofilm with varied grade. Results showed that 50% of the isolates were moderate biofilm producers. Genotypic analysis of biofilm related genes demonstrated that 78.6% of isolates harbored at least one of the genes tested.
Macro restriction analysis revealed 13 different patterns. Overall, the S. uberis isolates with the same PFGE patterns showed different biofilm related genes profiles. No correlation between the degree of biofilm produced and biofilm related genes profiles or Pulse Field Gel Electrophoresis patterns was found. Findings of the present study will contribute to improve the knowledge of this important pathogen in order to design appropriate strategies to improve the control and treatment of the disease. |
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Documentos electrónicos (1)
 Biofilm formation ability and genotypic analysis of Streptococcus uberis isolated from bovine mastitis Adobe Acrobat PDF |
